The painting has been in a private collection since the 1960s.
No. 210/No. 211 (Orange), painted in 1960, is a work from Rothko's most sought-after period, featuring large rectangles of color.
Rothko (1903-1970) is one of the most important and influential painters of the Abstract Expressionist movement, when American artists made their first major impact on the international art world.
At the height of his career, Rothko eliminated all representation in his paintings and focused on creating color abstractions aimed at eliciting an emotional response in his viewers.
The work is part of an exhibition titled See the Light: The Luminist Tradition in American Art, opening Oct. 13 and continuing through Jan. 28. Admission is free.
At the close of the exhibition, No. 210/No. 211 (Orange) will be relocated to one of the galleries housing Crystal Bridges' permanent collection.